Universal calibration slide PS20

Calibration of microscopes and image analysis systems is becoming more sophisticated, with the requirement being for a variety of image patterns to satisfy the numerous parameters. We're now introducing a new multi-function calibration standard specifically for these applications.Multiple images on a slide provide the most cost-effective solution to calibration and resolution checking of microscopes and image analysis systems. The combination of scales, dots, circles, squares, rulings, grids and angles can be supplied with an internationally traceable certificate of calibration for those who require ISO conformity.Each glass slide has a permanent serial number and can be supplied with full or partial UKAS certificate of accuracy.Starting from a fixed 'Datum Point' mark, each individual pattern or array can be located using X, Y, coordinates.General SpecificationGeneral tolerance (microns)

Calibration of microscopes and image analysis systems is becoming more sophisticated, with the requirement being for a variety of image patterns to satisfy the numerous parameters. We're now introducing a new multi-function calibration standard specifically for these applications.

Multiple images on a slide provide the most cost-effective solution to calibration and resolution checking of microscopes and image analysis systems. The combination of scales, dots, circles, squares, rulings, grids and angles can be supplied with an internationally traceable certificate of calibration for those who require ISO conformity.

Each glass slide has a permanent serial number and can be supplied with full or partial UKAS certificate of accuracy.

Starting from a fixed 'Datum Point' mark, each individual pattern or array can be located using X, Y, coordinates.

General Specification

General tolerance (microns)

Feature size ≤10µm 10 - 50µm 50 - 127µm 127 - 250µm >250µm
Tolerance 0.5 1.0 1.3 1.9 2.54
Coating Enduring evaporated chrome image
Optical density >2.5.
Substrate Soda lime glass
Size 76mm x 25mm x 1.5mm
Package Polished wooden case


PS20 Universal Calibration Slide Image Details

ID Pattern Name Location Description
A* Concentric Circles X =2;
Y = 10
1, 2, 3, 4, 5mm Circles with Cross Line & circle identifier. Line width 20µm
B* Concentric Squares X=10;
Y=10
1, 2, 3, 4, 5mm Squares with Cross Line & circle identifier. Line width 20 nm
C* Line Grating X=18;
Y=10
12.5 Line Pairs permm (40µm line 40µm space)
D* Line Grating,  X=26;
Y=10
50 Line Pairs permm (10µm line 10µm space)
E* Half Protractor X=34;
Y=10
15 Spacing, Line width 20µm
F* Grid Array Coarse X=40;
Y=10
5mm square array with 0.5mm divisions and central 2mm square with 0.25mm divisions. Line width 20µm
G* Grid Array Fine X=48;
Y=10
5mm square array with 0.1mm divisions and central 2mm square with 0.05mm divisions. Line width 8µm divisions and central 2mm square with 0.05mm divisions. Line width 8µm
H* Dot Array X=56;
Y=10
Dot diameter 0.25mm, dot centre to centre spacing 0.050mm - 11x11 grid = 121 dots to centre spacing 0.050mm - 11x11 grid = 121 dots
I* Geometric progression of Opaque Dots X=2;
Y=4
Line array of dots or square shapes, of either clear or opaque. Reducing in size in a Root 2 progression for the purposes of edge threshold detection to enable an image analyzer to measure the size correctly, or general shape size comparison.
J* Geometric X=17;
Y=4
Root 2 progression of 21 dots or square shapes, from 3.5µm to 3.5µm.
K* Geometric progression of Clear Dots X=32;
Y=4
Nominal size inmm
L* Geometric progression of Clear Squares X=47;
Y=4
Dot/square size - Large to small inmm 3.5833; 2.5338; 1.7917; 1.2669; 0.8959; 0.6335; 0.4479; 0.3167; 0.2240; 0.1584; 0.1120; 0.0792; 0.0560; 0.0396; 0.0280; 0.0198; 0.0140; 0.0099; 0.0070; 0.0049; 0.0035
M* Vertical Scale Fine Variable X=63 Overall Scale length 10mm.
5mm in 0.5mm divisions. Line width 20µm
4mm in 0.1mm divisions. Line width 10µm
1mm in 0.01mm divisions. Line width 3 µm
N* Horizontal  X=0;
Y=0
Scale length 62mm in 2mm
Scale Coarse divisions, subdivided in 1mm divisions with a 20µm line width.

*If you require a calibration certificate for any or all of the points A-N, please state which points and we will send you a quote.

Code Title Pack Size Availability Price Updated: 05-10-2024
EMS68053-20 Universal calibration slide PS20 Each See above $860.00 AUD