Tris buffer (10x)

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

Storage: 2-8°C. DO NOT FREEZE 
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: 
Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

Technical data sheet

SDS

Code Title pH Pack Size Availability Price Updated: 21-05-2024
EMS64142-11 Tris buffer (10x) 10 100ml See above $180.00 AUD
EMS64142-12 Tris buffer (10x) 10 1L See above $303.00 AUD