Citifluor antifadent, CFPVOH plus AF100

Antifadent solutions reduce the photobleaching or fading of the fluorescence of dyes used for labelling biological species. The fading of fluorochrome dyes is a particular problem in fluorescence microscopy, such as in immunofluorescence studies. Fluorescent dyes are also used as cell markers, for following the uptake and release of calcium from cells for example, and for characterising cell surfaces. on-fluorescent immersion oil is available for work at high magnifications.

Things to consider when selecting the correct antifadent:

1. Do you need a Hardening or a Non Hardening medium?
2. If your system is Glycerol tolerant than choose a Glycerol Based product, if not then use a Glycerol Free solution
3. These high refractive index mountant solutions are to be used where you wish to obviate the effects of spherical aberration (caused by a mismatch between the refractive index of the glass of the coverslip and the mountant medium) which leads to a loss of resolution of your images

Choosing and using the best antifadent for your application: Technical Data

Store at room temperature

Non-Hardening Mountant - Glycerol Free

• AF100 - Safety Data Sheet

Hardening Mountants - Film Forming Polymer Solutions

• CFPVOH - Safety Data Sheet