LR White resin only, uncatalysed, (DG)
A very low viscosity (8cps), non-toxic resin, suitable for both light and electron microscopy. LR White is a polar monomer polyhydroxylated achromatic acrylic resin. Curable by heat or light. Sections of polymerised LR White resin are hydrophilic. This characteristic allows immunocytochemistry reagents and stains to easily penetrate the sections. Etching of sections is not necessary. LR White sections show minimal non-specific staining.
Its low viscosity makes it ideal for infiltrating decalcified bone and teeth as well as plant tissue. It is available in medium and hard grade.
Distance, time and high temperatures during shipping from the manufacturer would age catalysed resin too much, we purchase and ship LR White uncatalysed, but the material is supplied with catalyst (benzoyl peroxide) and detailed instructions, but read in the linked User Notes how to catalyse without ruining the whole bottle. Catalysed LR White keeps refrigerated for over one year and embedded specimens can be cured using heat only. For cold curing with light, accelerator must be added.
Newman, G.R. Use and abuse of LR White. Histochem.J. 19:118 (1987)
Newman, G.R. LR White embedding medium for colloidal gold method. In: Colloidal Gold: Principles, Methods, and Applications, Vol.2 (Hayat, M.A., Ed.). Academic Press, San Diego and London (1989).
Note: Minor acid number variation of some resins results in a colour change when the mixture is made up: from clear to amber to pink. This does not affect resin performance.
Store refrigerated 0-5°C
Benzoyl peroxide Safety Data Sheet
C024C is catalysed and needs to be shipped cold from Townsville, preferably no further than Sydney. It is not a DG.