Hollande's fixative (DG)

A variant of Bouin's solution. It is stable and capable of decalcifying small bone specimens. Tissue fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilises red blood cell membranes as well as cosinophil and endocrine cell granules, resulting in less lysis than with Bouin's solution. Hollande's is commonly used as a fixative for gastrointestinal biopsy tissues.Because the salts in the solution will produce an insoluble phosphate precipitate, it is necessary to thoroughly wash the fixative prior to placing the specimen in a phosphate buffered formalin solution. Prefilled ready-to-use histo-containers are packaged in leak-proof histo-containers.Store at room temperature

A variant of Bouin's solution. It is stable and capable of decalcifying small bone specimens. Tissue fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilises red blood cell membranes as well as cosinophil and endocrine cell granules, resulting in less lysis than with Bouin's solution. Hollande's is commonly used as a fixative for gastrointestinal biopsy tissues.

Because the salts in the solution will produce an insoluble phosphate precipitate, it is necessary to thoroughly wash the fixative prior to placing the specimen in a phosphate buffered formalin solution. Prefilled ready-to-use histo-containers are packaged in leak-proof histo-containers.

Store at room temperature

Code Cup Size Filled
EMS64120-10 15ml 10ml
EMS64120-20 30ml 20ml
EMS64120-30 60ml 30ml
EMS64120-01
1000ml
EMS64120-04
4000ml

MSDS

Updated: 06-12-2024
Code Title Volume Pack Size Availability Price Updated: 06-12-2024
EMS64120-01 Hollande's fixative (DG) 1L Each 0 in stock (?) $152.00 AUD
EMS64120-04 Hollande's fixative (DG) 4L Each 0 in stock (?) $433.00 AUD
EMS64120-10 Hollande's fixative (DG) 10ml Pack/50 0 in stock (?) $179.00 AUD
EMS64120-20 Hollande's fixative (DG) 20ml Pack/50 0 in stock (?) $234.00 AUD
EMS64120-30 Hollande's fixative (DG) 30ml Pack/20 0 in stock (?) $124.00 AUD