Trypsin kit
  • Collections

Trypsin kit

Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.Storage: 2-8°C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 mlLarge: Reagent B (Buffer) is 100 ml and...

Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.

Storage: 2-8°C. DO NOT FREEZE 

Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:

Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml

Technical data sheet

SDS Reagent B

SDS Reagent E

Code Title Size Pack Size Availability Price Updated: 13-09-2024
EMS64142-01 Trypsin kit Small Kit See above $179.00 AUD
EMS64142-02 Trypsin kit Large Kit See above $326.00 AUD