R-Buffer

For use in antigen unmasking and correction of overfixed tissue.  To improve the morphology of tissue of the processed sections, use one of our supplied ready-to-use buffers by choosing them according to pH.Antigen RetrieverBench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining. Ensures identical processing of all samples during a processing cycle and preserves processed tissues.Technical Data Sheet

For use in antigen unmasking and correction of overfixed tissue.  To improve the morphology of tissue of the processed sections, use one of our supplied ready-to-use buffers by choosing them according to pH.

Picture of Antigen Retriever

Antigen Retriever

Bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining. Ensures identical processing of all samples during a processing cycle and preserves processed tissues.

Technical Data Sheet

Code Description pH MSDS
EMS62706-10 R-Buffer A
(10X)
6.0 MSDS
EMS62706-11 R-Buffer B
(10X)
8.0 MSDS
EMS62706-12 R-Buffer C
(10X, CLASS 6.1 PG II)
4.5 MSDS
EMS62706-13 R-Buffer U
(10X)
6.0 MSDS
EMS62707-10 R-Buffer Ag
(2X)
6.0 MSDS
EMS62707-11 R-Buffer Bg
(2X)
8.0 MSDS
EMS62707-12 R-Buffer Cg
 (2X)
4.5 MSDS
EMS62707-13 R-Buffer Ug
(2X)
6.0 MSDS

Choosing Buffer: Correction for the Nature of the Antigen and Epitope

The choice of the buffer depends on the nature of the antigen and the location of the epitope of interest. We advise you to first run the test for the most appropriate buffer using the tissues where the expression of the antigen does occur. General guidelines for choosing the buffer:

Most of the nuclear antigens (apoptosis-related, survival-related, proliferation-related) R: Buffer A (or AG).

Cell adhesion molecules, cell membrane antigens (extracellular domain) R: Buffer A (or AG)

Cytoskeleton and cytoskeleton-associated molecules R: Buffer A (or AG)

Intracellular domain of some adhesion molecules and surface receptors R: Buffer B (or BG)

Intracellular domain of some adhesion molecules and surface receptors R: Buffer C (or CG)

Most of antibodies raised against a linear peptide R: Buffer U (or UG)

Code Title pH Pack Size Availability Price Updated: 24-04-2024
EMS62706-10 R-Buffer 6.0 (A) 250ml See above $172.00 AUD
EMS62706-11 R-Buffer 8.0 (B) 250ml See above $172.00 AUD
EMS62706-12 R-Buffer 4.5 (C) 250ml See above $172.00 AUD
EMS62706-13 R-Buffer 6.0 (U) 250ml See above $172.00 AUD
EMS62707-10 R-Buffer 6.0 (Ag) 250ml See above $172.00 AUD
EMS62707-11 R-Buffer 8.0 (Bg) 250ml See above $172.00 AUD
EMS62707-12 R-Buffer 4.5 (Cg) 250ml See above $172.00 AUD
EMS62707-13 R-Buffer 6.0 (Ug) 250ml See above $172.00 AUD