Phosphotungstic acid, aqueous

(Tungstophosphoric Acid)H3[P(W3O10)4]   aq F.W. 2880.17+aq   CAS #12501-23-4Specifications:

(Tungstophosphoric Acid)

H3[P(W3O10)4]   aq F.W. 2880.17+aq   CAS #12501-23-4


Appearance   Practically clear, colorless solution.
Ammonia (NH )   0.004%
Chloride (Cl)   0.03%
Insoluble Matter   0.02%
Nitrate (NO3 )   0.004%
Sulfate (SO4 )   0.005%
Heavy Metals and Iron.   No color formation onaddition of Hydrogen Sulfide

Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.

Used widely as a Negative Stain:

  • As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
  • Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
  • Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
  • Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
  • Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
Code Title Percentage Pack Size Availability Price Updated: 09-12-2021
EMS19502-1 Phosphotungstic acid, aqueous 1% 500mL 2 weeks $97.00 AUD
EMS19502-3 Phosphotungstic acid, aqueous 3% 500mL 2 weeks $117.00 AUD
EMS19502-5 Phosphotungstic acid, aqueous 5% 500mL 2 weeks $147.00 AUD