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Karnovsky's fixative

Paraformaldehyde-Glutaraldehyde Karnovsky's FixativeKit contains2 x 10 ml 16% Paraformaldehyde Solution1 x 10 ml 50% EM Grade Glutaraldehyde1 x 50 ml Sodium Phosphate Buffer .2M, pH 7.2_____________________________________________________________________________Mixture 1 (Original Karnovsky's Mixture)

Paraformaldehyde-Glutaraldehyde Karnovsky's Fixative

Kit contains

  • 2 x 10 ml 16% Paraformaldehyde Solution
  • 1 x 10 ml 50% EM Grade Glutaraldehyde
  • 1 x 50 ml Sodium Phosphate Buffer .2M, pH 7.2

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Mixture 1 (Original Karnovsky's Mixture)

16% Paraformaldehyde Solution 20 ml
50% Glutaraldehyde EM Grade 8 ml
0.2M Sodium Phosphate Buffer 25 ml
Distilled Water 25 ml

Final mixture is 78 ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.064M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .

Mixture 2

A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.

16% Paraformaldehyde Solution 13 ml
50% Glutaraldehyde EM Grade 5 ml
0.2M Sodium Phosphate Buffer 50 ml
Distilled Water 32 ml

Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.

Code Title Pack Size Availability Price Updated: 05-12-2021
EMS15720 Karnovsky's fixative Kit 2 weeks $67.00 AUD