Fluoro-Gel III, water-based mounting medium

Fluoro-Gel III mounting medium is similar to Fluoro-Gel. However, the addition of PI (Propidium Iodide), which is a counterstain for DNA, makes this a unique mounting medium, which prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC).The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain Phynylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC, and APC. This product is to be used in in situ hybridisation techniques or other method where fluorescence of DNA staining is required. PI excites at 535nm, emits at 615nm, producing a red fluorescence. RNA is also stained with PI.Application: Immunofluorescence, confocal microscopyStorage: 2-8°DO NOT FREEZE

Fluoro-Gel III mounting medium is similar to Fluoro-Gel. However, the addition of PI (Propidium Iodide), which is a counterstain for DNA, makes this a unique mounting medium, which prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC).
The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain Phynylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC, and APC. This product is to be used in in situ hybridisation techniques or other method where fluorescence of DNA staining is required. PI excites at 535nm, emits at 615nm, producing a red fluorescence. RNA is also stained with PI.

Application: Immunofluorescence, confocal microscopy
Storage: 2-8°DO NOT FREEZE

Technical Data Sheet

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