EDTA buffer (10X)

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

Storage: 2-8°C. DO NOT FREEZE 
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 8.5 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

Technical data sheet


Code Title pH Pack Size Availability Price Updated: 22-05-2024
EMS64142-09 EDTA buffer (10X) 8.5 100ml See above $169.00 AUD
EMS64142-10 EDTA buffer (10X) 8.5 1L See above $281.00 AUD