Citifluor antifadent solutions

Antifadent solutions reduce the photobleaching or fading of the fluorescence of dyes used for labelling biological species. The fading of fluorochrome dyes is a particular problem in fluorescence microscopy, such as in immunofluorescence studies. Fluorescent dyes are also used as cell markers, for following the uptake and release of calcium from cells for example, and for characterising cell surfaces. Non-fluorescent immersion oil is available for work at high magnifications.Things to consider when selecting the correct antifadent:Do you need a Hardening or a Non Hardening medium?If your system is Glycerol tolerant than choose a Glycerol Based product, if not then use a Glycerol Free solutionThese high refractive index mountant solutions are to be used where you wish to obviate the effects of spherical aberration (caused by a mismatch between the refractive index of the glass of the coverslip and the mountant medium) which leads to a...

Antifadent solutions reduce the photobleaching or fading of the fluorescence of dyes used for labelling biological species. The fading of fluorochrome dyes is a particular problem in fluorescence microscopy, such as in immunofluorescence studies. Fluorescent dyes are also used as cell markers, for following the uptake and release of calcium from cells for example, and for characterising cell surfaces. 

Non-fluorescent immersion oil is available for work at high magnifications.

Things to consider when selecting the correct antifadent:

  1. Do you need a Hardening or a Non Hardening medium?
  2. If your system is Glycerol tolerant than choose a Glycerol Based product, if not then use a Glycerol Free solution
  3. These high refractive index mountant solutions are to be used where you wish to obviate the effects of spherical aberration (caused by a mismatch between the refractive index of the glass of the coverslip and the mountant medium) which leads to a loss of resolution of your images

Guide to Non-Hardening Mountants
Glycerol Based
AF1 - A glycerol-PBS solution contains an amine antifadent
AF1 plus DAPI - contains both an amine antifadent and the DNA stain, DAPI
AF2 A glycerol solution contains an amine antifadent that allows you to choose your own buffer solution
AF4 - A glycerol solution of n-propyl gallate

Choosing and using the best antifadent for your application: Technical Data

Store at room temperature

Non-Hardening Mountant - Glycerol Based:

AF1 -  Safety Data Sheet

AF1

AF2 -  Safety Data Sheet

AF4 

Non-Hardening Mountant - High Refractive Index Glycerol Based Solutions:

CFM-1 - Safety Data Sheet

CFM-1 plus AF - Safety Data Sheet

CFM-2 - Safety Data Sheet

CFM-3 - Safety Data Sheet

CFMR2 -  Safety Data Sheet

Non-Hardening Mountant - Glycerol Free:

AF3 -  Safety Data Sheet

CFPVOH 

AF100 -  Safety Data Sheet

AFR3 -  Safety Data Sheet

Non-Hardening Mountant - High Refractive Index Glycerol Free Solutions:

AF87 - Safety Data Sheet

Hardening Mountants - Glycerol Based:

AF200 -  Safety Data Sheet

AF300 -  Safety Data Sheet

AFR3 -  Safety Data Sheet

Hardening Mountants - Film Forming Polymer Solutions:

Tris-MWL 4-88 -  Safety Data Sheet

CFPVOH - Safety Data Sheet

Hardening Mountants - Glycerol Free:

Updated: 08-12-2024
Code Title Type Pack Size Availability Price Updated: 08-12-2024
EMS17970-100 Citifluor antifadent solutions AF1 100ml 0 in stock (?) $196.00 AUD
IAF1-D-15 Citifluor antifadent solutions AF1 + DAPI 15ml 0 in stock (?) $86.00 AUD
IAF2-100 Citifluor antifadent solutions AF2 100ml 0 in stock (?) $196.00 AUD
IAF3-100 Citifluor antifadent solutions AF3 100ml 0 in stock (?) $196.00 AUD
IAFR3-10 Citifluor antifadent solutions AFR3 10ml 0 in stock (?) $123.00 AUD
IAF4-25 Citifluor antifadent solutions AF4 25ml 0 in stock (?) $91.00 AUD
IAF87-25 Citifluor antifadent solutions AF87 25ml 0 in stock (?) $121.00 AUD
IAF200-10 Citifluor antifadent solutions AF200 10ml 0 in stock (?) $86.00 AUD
IPVP1-A-25 Citifluor antifadent solutions PVP-1 + Antifadent 25ml 0 in stock (?) $86.00 AUD
IMWL4-88-25 Citifluor antifadent solutions MWL4-88 25ml 0 in stock (?) $91.00 AUD
IAF300-10 Citifluor antifadent solutions AF300 10ml 0 in stock (?) $86.00 AUD
IPVP1-25 Citifluor antifadent solutions PVP-1 25ml 0 in stock (?) $91.00 AUD
ICFMR2-10 Citifluor antifadent solutions CFMR2 10ml 0 in stock (?) $99.00 AUD
ICFM-1-10 Citifluor antifadent solutions CFM-1 10ml 0 in stock (?) $73.00 AUD
ICFM-1-25 Citifluor antifadent solutions CFM-1 25ml 0 in stock (?) $115.00 AUD
ICFM-1A-10 Citifluor antifadent solutions CFM-1+Antifadent 10ml 0 in stock (?) $74.00 AUD
ICFM-1A-25 Citifluor antifadent solutions CFM-1+Antifadent 25ml 0 in stock (?) $124.00 AUD
ICFM-2-10 Citifluor antifadent solutions CFM-2 10ml 0 in stock (?) $73.00 AUD
ICFM-2-25 Citifluor antifadent solutions CFM-2 25ml 0 in stock (?) $113.00 AUD
IAF100-5 Citifluor antifadent solutions AF100 5ml 0 in stock (?) $30.00 AUD
IAF1-25 Citifluor antifadent solutions AF1 25ml 0 in stock (?) $89.00 AUD
IAF2 Citifluor antifadent solutions AF2 25ml 0 in stock (?) $91.00 AUD
IAF3 Citifluor antifadent solutions AF3 25ml 0 in stock (?) $91.00 AUD
IAF87 Citifluor antifadent solutions AF87 10ml 0 in stock (?) $77.00 AUD
ICFM3-10 Citifluor antifadent solutions CFM3 10ml 0 in stock (?) $72.00 AUD
ICFM3-25 Citifluor antifadent solutions CFM3 25ml 0 in stock (?) $115.00 AUD