C-flat holey carbon coated grids for cryo-TEM

C-flat is an ultra-flat, holey carbon-coated TEM support grid for transmission electron microscopy (TEM). Unlike competing holey carbon films, C-flat is manufactured without plastics, so it is clean upon arrival and the user has no residue to contend with.

C-flat, a product of patent-pending technology, offers an ultra-flat surface that improves particle dispersion and produces more consistent ice thickness. Deep-UV projection lithography is used for patterning, which guarantees the most precise and reliable hole sizes and shapes down to submicron features. The precise methods by which C-flat is manufactured eliminate artefacts such as excess carbon and edges around holes.

Applications:

C-flat holey carbon grids provide the ideal specimen support to achieve high resolution data in cryo-TEM making them an ideal choice for single particle analysis, cryo electron tomography and automated TEM analysis.

Cryo-Electron Tomography (cryoET) and Single Particle Analysis (SPA):

Numerous researchers have reported that the ultra-flat surface of C-flat leads to even ice thickness and uniform particle distribution within the hole areas. This optimal particle distribution results in superior data being collected as compared with other holey support films.  2um hole sizes are standard.

Technical Data