Correlative microscopy coverslips (EMS)

EMS introduces a simple coverslip with a grid image that allows cells to grow and later be analysed under brightfield or fluorescence microscopy before being further processed and sectioned for EM or other analysis. These unique coverslips do all that.

Applications:

Light Microscopy, Fluorescence, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), High-Pressure Cryofixation, Reticles in microscope eyepieces, Calibration scale/grid on the microscope stage, Measurement tool when using magnifiers , Cover glass to make direct measurements, Specimen support 

All of the coverslips are produced on a polyester based film, 0.18 mm thick, 22 x 22 mm, 25/box. All other grid sizes and film sizes are available upon special order.

Features:

• Reticle film has been thoroughly tested with cell culture
• Good growth of cell culture (equivalent to conventional media)
• Good adhesion to the substrate without cell polylysine
• Resistant to normal chemicals used in electron microscopy
• No oxygen retention, compatible resin LR White
• Good optical quality in brightfield & UV fluorescence
• Excellent transparency
• Does not deform at temperatures (positive 100?C and negative liquid N2)
• Rigid, does not float in the middle of culture
• Easy to handle and cut with a knife or micro-punch
• Simple sterilisation using alcohol or UV
• Detaches easily from resin after polymerisation
• Low cost

User Notes