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Citifluor antifadent solutions
Antifadent solutions reduce the photobleaching or fading of the fluorescence of dyes used for labelling biological species. The fading of fluorochrome dyes is a particular problem in fluorescence microscopy, such as in immunofluorescence studies. Fluorescent dyes are also used as cell markers, for following the uptake and release of calcium from cells for example, and for characterising cell surfaces.
Non-fluorescent immersion oil is available for work at high magnifications.
Things to consider when selecting the correct antifadent:
- Do you need a Hardening or a Non Hardening medium?
- If your system is Glycerol tolerant than choose a Glycerol Based product, if not then use a Glycerol Free solution
- These high refractive index mountant solutions are to be used where you wish to obviate the effects of spherical aberration (caused by a mismatch between the refractive index of the glass of the coverslip and the mountant medium) which leads to a loss of resolution of your images
Guide to Non-Hardening Mountants
Glycerol Based
AF1 - A glycerol-PBS solution contains an amine antifadent
AF1 plus DAPI - contains both an amine antifadent and the DNA stain, DAPI
AF2 A glycerol solution contains an amine antifadent that allows you to choose your own buffer solution
AF4 - A glycerol solution of n-propyl gallate
Choosing and using the best antifadent for your application: Technical Data
Store at room temperature
Non-Hardening Mountant - Glycerol Based:
AF1 - Safety Data Sheet
AF1
AF2 - Safety Data Sheet
AF4
Non-Hardening Mountant - High Refractive Index Glycerol Based Solutions:
CFM-1 - Safety Data Sheet
CFM-1 plus AF - Safety Data Sheet
CFM-2 - Safety Data Sheet
CFM-3 - Safety Data Sheet
CFMR2 - Safety Data Sheet
Non-Hardening Mountant - Glycerol Free:
AF3 - Safety Data Sheet
CFPVOH
AF100 - Safety Data Sheet
AFR3 - Safety Data Sheet
Non-Hardening Mountant - High Refractive Index Glycerol Free Solutions:
AF87 - Safety Data Sheet
Hardening Mountants - Glycerol Based:
AF200 - Safety Data Sheet
AF300 - Safety Data Sheet
AFR3 - Safety Data Sheet
Hardening Mountants - Film Forming Polymer Solutions:
Tris-MWL 4-88 - Safety Data Sheet
CFPVOH - Safety Data Sheet
Hardening Mountants - Glycerol Free:
- PVP plus anti-fadent - Safety Data Sheet
- CFPVOH plus anti-fadent
Code | Type | Pack Size | Availability | Price | Updated: 04-04-2025 |
|---|---|---|---|---|---|
| IAF1-25 | AF1 | 25ml | 0 in stock (?) | $96.00 AUD | |
| EMS17970-100 | AF1 | 100ml | 0 in stock (?) | $196.00 AUD | |
| IAF2 | AF2 | 25ml | 0 in stock (?) | $91.00 AUD | |
| IAF2-100 | AF2 | 100ml | 0 in stock (?) | $196.00 AUD | |
| IAF3 | AF3 | 25ml | 0 in stock (?) | $91.00 AUD | |
| IAF3-100 | AF3 | 100ml | 0 in stock (?) | $196.00 AUD | |
| IAF4-25 | AF4 | 25ml | 0 in stock (?) | $91.00 AUD | |
| IAF87 | AF87 | 10ml | 0 in stock (?) | $77.00 AUD | |
| IAF87-25 | AF87 | 25ml | 0 in stock (?) | $121.00 AUD | |
| ICFM-1-10 | CFM-1 | 10ml | 0 in stock (?) | $73.00 AUD | |
| ICFM-1-25 | CFM-1 | 25ml | 0 in stock (?) | $115.00 AUD | |
| ICFM-1A-10 | CFM-1+Antifadent | 10ml | 0 in stock (?) | $74.00 AUD | |
| ICFM-1A-25 | CFM-1+Antifadent | 25ml | 0 in stock (?) | $124.00 AUD | |
| ICFM-2-10 | CFM-2 | 10ml | 0 in stock (?) | $73.00 AUD | |
| ICFM-2-25 | CFM-2 | 25ml | 0 in stock (?) | $113.00 AUD | |
| IAF1-D-15 | AF1 + DAPI | 15ml | 0 in stock (?) | $86.00 AUD | |
| IAFR3-10 | AFR3 | 10ml | 0 in stock (?) | $123.00 AUD | |
| IAF100-5 | AF100 | 5ml | 0 in stock (?) | $30.00 AUD | |
| IAF200-10 | AF200 | 10ml | 0 in stock (?) | $86.00 AUD | |
| IAF300-10 | AF300 | 10ml | 0 in stock (?) | $86.00 AUD | |
| IPVP1-25 | PVP-1 | 25ml | 0 in stock (?) | $91.00 AUD | |
| IPVP1-A-25 | PVP-1 + Antifadent | 25ml | 0 in stock (?) | $86.00 AUD | |
| IMWL4-88-25 | MWL4-88 | 25ml | 0 in stock (?) | $91.00 AUD | |
| ICFMR2-10 | CFMR2 | 10ml | 0 in stock (?) | $99.00 AUD | |
| ICFM3-10 | CFM3 | 10ml | 0 in stock (?) | $72.00 AUD | |
| ICFM3-25 | CFM3 | 25ml | 0 in stock (?) | $115.00 AUD |
* Ordering Schedule
This item is available for back-order where 0 stock is listed.
We order from this manufacturer weekly, with an estimated lead time of 4 weeks.
This is the estimated lead time, based on the average time taken previously to fill orders from this particular supplier.
Lead times are from when we receive your order until when we fill your order. For delivery times, please see shipping information below.
