JB-4 embedding kit (DG)

The JB-4 EMBEDDING KIT is a water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalysed monomer acts as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4. Features:

Thin sections (0.5-2.0 micron) with excellent morphological structure preservation. Water-clear blocks; casts in 90 minutes max. at room temperature. Good lipid and enzyme retention when processing at low temperatures (4?C). Removal of JB-4 resin prior to staining is not necessary. Clearing agents such as xylene and chloroform are not needed. Higher clarity and contrast than with paraffin sections. Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue. 

Technical Data

All components may be stored at room temperature.

JB-4 kit components:

C611 JB-4 solution A only, 750ml

C612 JB-4 solution B only 50ml

C613 JB-4 catalyst 12g

References: 1. Hofman E.O. and Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981) 2. Beckstead, J.H., Blood, 57(6):1008 (1981) 3. Brinn, N and Pickett, P., J. Histotech., 2(5):125-130 (1979) 4. Block, Matthew H., et al., Lab. Med., 13(5)(1982) 5. Helander, K.G., J. Microscopy, 132:223 (1983) 6. Cole, M.B., J. Microscopy, 127:139 (1982) 7. Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979) 8. Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980) 9. Horton, W.A., J. Histochem. Cytochem., 31:417 (1983) 10. Tacha, D.E. and Richard, T.C.., J. Histotech., 4(2):59 (1981)

Solution A Safety Data Sheet

Solution B Safety Data Sheet

Catalyst Safety Data Sheet