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Perfect loop set, ultra thin
The Perfect Loop is used to easily place ultra-thin sections on specimen grids without folds. It is the only loop presently offered where the loop's external diameter is the same as the grid, and the internal diameter is slightly bigger than the electron microscopes observational area.The thickness is roughly 40 microns. Because the loop and the grid have the very same diameter they are attracted with each other when in water and fasten together through the surface tension of the water.
However if the section hits the inside of the grid while the touching area is being blotted is minor and therefore the section is not harmed. Upon removal of the grid from the loop the section remains intact without fail. The area equals the electron microscopes observation field (about 2mm diameter); therefore pieces can be adequately identified.
Perfect Loop for Ultra Thin sections | |||
1. Center the loop above the sections |
2. Slowly lower the loop over the sections and touch the water |
3. Gently lift up the loop with the sections in a droplet of water |
4. Lower the loop onto a grid and lift up again |
5. The grid holds to the loop by surface tension |
6. Lower the loop to the filter paper to remove water |
7. For coated grids, touch with filter paper to remove water |
8. Separate the grid from the loop with an eyelash |