Aurion anionic gold tracer

Aurion Anionic Gold Tracers are used to visualise charged moieties in specimens. They bind to polycationic moieties (basic proteins e.g. histones). "Neutral" BSA coated gold tracers are useful for detecting open connections and tissue damage. Under proper conditions and with suitable microscopical techniques, the tracers can also be used to follow cellular events in time.

Application Instructions 

In order to obtain a positive reaction when using these tracers the following should be kept in mind: charge interactions are influenced by the presence of ions, di- and trivalent ions being of more influence than univalent ions. The influence is concentration dependent. Binding is stronger in low ionic-strength media. charge interactions are influenced by pH. The charge of components in the specimen is 

The AURION Gold Tracers are available in the full range of particle sizes: Ultra Small, 6, 10, 15 and 25nm. Anionic Gold Tracers are prepared with BSA-c as particle conjugated protein.

• Anionic and BSA tracers
• Available with conventional particle sizes
• Available with ultra small particle sizes
• For the microscopical detection of: charged moieties, open connections and tissue damage, cellular dynamic events (event tracking)

AURION Gold Tracers are available in 5 and 10ml volume packages and are supplied at an OD520nm of 2.0 for the conventional particle size range and at equivalent OD for the Ultra Small tracers.

The products are supplied in PBS, with 15mM NaN₃.

Note 1:
AURION Gold Tracers are also available in bulk and at different optical density if required.

Note 2:
AURION Gold Tracers are shipped containing NaN₃ as preservative. If they are intended for use in living organisms, the preservative has to be removed prior to use. This can be achieved either by dialysis or by buffer exchange using for instance a GE Healthcare Life Sciences PD-10 column.

Store refrigerated 4-8°C

MSDS