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Tris buffer (10x)
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZEÂ
Intended Use:Â 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
Code | Title | pH | Pack Size | Availability | Price | Updated: 20-05-2024 |
---|---|---|---|---|---|---|
EMS64142-11 | Tris buffer (10x) | 10 | 100ml | See above | $180.00 AUD | |
EMS64142-12 | Tris buffer (10x) | 10 | 1L | See above | $303.00 AUD | |