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Carstairs fibrin and platelet staining, picric acid (DG)
Picric acid stain solution (reagent grade) is used in Carstairs method for Fibrin and platelets. 2,4,6-TrinitrophenolFormalin-Saline for 48 hours or moreStaining Procedures:Hydrate slides to distilled water.Mordant in Ferric Ammonium Sulfate, 5% (EMS26381-01) for 5 minutes. Rinse in running tap water.Stain with Mayer Hematoxylin (EMS26381-02) for 5 minutes. Rinse in running tap water.Stain in the Picric Acid-Orange G Solution (EMS26381-03) for 30 minutes to 1 hour. Rinse once in distilled water.Stain in Ponceau Fuchsin Solution (EMS26381-04) for 1 to 5 minutes. Rinse in distilled water.Differentiate with Phosphotungstic Acid, 1% (EMS26381-05) until the muscle is red and background pale pink. Rinse in distilled water.Stain with Aniline Blue Solution (EMS26381-06) for 30 minutes to 2 hours. Rinse in several changes of distilled water.
Picric acid stain solution (reagent grade) is used in Carstairs method for Fibrin and platelets.
2,4,6-Trinitrophenol
Formalin-Saline for 48 hours or more
Staining Procedures:
- Hydrate slides to distilled water.
- Mordant in Ferric Ammonium Sulfate, 5% (EMS26381-01) for 5 minutes. Rinse in running tap water.
- Stain with Mayer Hematoxylin (EMS26381-02) for 5 minutes. Rinse in running tap water.
- Stain in the Picric Acid-Orange G Solution (EMS26381-03) for 30 minutes to 1 hour. Rinse once in distilled water.
- Stain in Ponceau Fuchsin Solution (EMS26381-04) for 1 to 5 minutes. Rinse in distilled water.
- Differentiate with Phosphotungstic Acid, 1% (EMS26381-05) until the muscle is red and background pale pink. Rinse in distilled water.
- Stain with Aniline Blue Solution (EMS26381-06) for 30 minutes to 2 hours. Rinse in several changes of distilled water.
- Dehydrate, clear in Xylene and coverslip, using a synthetic mounting medium.
Stain Results
Fixation Time: | 48 Hours or more | Less than 48 hours |
Fibrin | Bright Red | Orange to Orange Red |
Platelets | Gray Blue or Navy | Light Gray |
Collagen | Bright Blue | Bright Blue |
Muscle | Red | Red |
Red Blood Cells | Clear Yellow | Red, Green or Yellow |
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Code | Title | Pack Size | Availability | Price | Updated: 08-05-2024 | |
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C26381-03 | Carstairs fibrin and platelet staining, picric acid (DG) | 500ml | 2 in stock | $180.00 AUD | ||