Aurion silver enhancement reagents, light microscopy

The AURION R-GENT SE-INTENSE components constitute a Silver Enhancement Reagent which increases the average gold cluster or particle size by deposition of metallic silver. The generated intense brown-black signal is compatible with counterstaining in light microscopy. The signal is also easily detected in bio assays. AURION R-GENT SE-INTENSE has been tailored for the enhancement of AURION Ultra Small and AURION 2nm Immunogold reagents and is equally suited for the larger sized particles in the AURION Conventional Immunogold reagents.

• For light microscopy and bio assays
• Improved signal intensity
• Light insensitive
• Virtually no autonucleation
• Improved reproducibility
• Dropper bottles, easy preparation
• Economical use, no leftovers

For enhancement, equal amounts of the DEVELOPER and ENHANCER are mixed well and applied to the specimen. The enhancement mixture is easy-to-use, exhibits extremely delayed auto-nucleation and can be used under standard laboratory light conditions. Typical enhancement times are between 15 and 25 minutes. Auto-nucleation becomes visible only after 35-45 minutes. Light microscopical specimens may be counterstained according to standard procedures. The enhancement mixture has a pH value of 8.3-8.5.

AURION R-GENT SE-INTENSE is available as a kit in two unit sizes (2 x 30ml or 2 x 250ml) and consists of a separate DEVELOPER and ENHANCER. The supplied amounts accomodate 600 and 5000 LM specimens respectively at 100µl/specimen, or 60 and 500 bio assay specimens at 1ml/specimen. The reactivity is tested on dot-spots and the absence of autonucleation is monitored by spectrophotometric techniques.

Storage:

The AURION R-Gent SE-EM components are stored at 4°C and allowed to reach room temperature before use. SE-EM INITIATOR may be stored at -20°C for prolonged shelf life

MSDS