DGD embedding kit (DG)

DGD is a removable embedding medium (for thin and thick sections) that provides embedment free sections. Good for immunolabeling and high resolution light microscopy.Advantages:Thin or thick embedment free sections can be obtained.Sections are easy to cut and ribbons are easily obtainable.Enhances the integrity of the specimen as it floats on the water.Sections produce interference colors according to its thickness.Ethanol and n-butyl alcohol are used as dehydrating agents.Capco, Krockmalnic, & Penman (1984). A New Method of Preparing Embedment Free Sections For TEM: Applications to the Cytoskeletal Framework and Other 3D Networks. JCB Vol. 98, pp. 1878.Valdimarsson & Huebner (1989). DEGDS as an Embedding Medium for Immunofluorescence Microscopy. Biochem. Cell Biol. 67:242.Nickerson, Krockmalnic, He, & Penman (1990). Immunolocalization in Three Dimensions: Immunogold Sta...

DGD is a removable embedding medium (for thin and thick sections) that provides embedment free sections. Good for immunolabeling and high resolution light microscopy.

Advantages:

  • Thin or thick embedment free sections can be obtained.
  • Sections are easy to cut and ribbons are easily obtainable.
  • Enhances the integrity of the specimen as it floats on the water.
  • Sections produce interference colors according to its thickness.

Ethanol and n-butyl alcohol are used as dehydrating agents.

  1. Capco, Krockmalnic, & Penman (1984). A New Method of Preparing Embedment Free Sections For TEM: Applications to the Cytoskeletal Framework and Other 3D Networks. JCB Vol. 98, pp. 1878.
  2. Valdimarsson & Huebner (1989). DEGDS as an Embedding Medium for Immunofluorescence Microscopy. Biochem. Cell Biol. 67:242.
  3. Nickerson, Krockmalnic, He, & Penman (1990). Immunolocalization in Three Dimensions: Immunogold Staining of Cytoskeletal and Nuclear Matrix Proteins in Resinless Electron Microscopy Sections. PNAS Vol. 87, pp. 2259.

Kit consists of:

  • 450mL Diethylene Glycol Distearate (DGD) (EMS13255) SDS 
  • 450mL Ethanol
  • 450mL n-Butyl alcohol (EMS26901-03)  SDS 

Technical Data Sheet 

Code Title Pack Size Availability Price Updated: 16-05-2021
EMS14010 DGD embedding kit (DG) Each 3 months $610.00 AUD
Code Title Pack Size Availability Price
EMS14010 DGD embedding kit (DG) Each 3 months $610.00 AUD