Superfrost plus gold microscope slide

These slides are specially designed for the application of fresh or formalin fixed frozen tissue. They are inherently tissue binding so there is no need to add sprays or adhesives to the slides.

Features:

• Dimensions: 25 x 75 x 1mm
• Securely attach frozen breast, brain, skin and other normally difficult to bond tissue sections to the slide
• Ideal when special stains, immunocytochemical and in situ DNA hybridisation techniques are applied on fresh and fixed frozen tissue
• Assist tissue adherence during immunocytochemical analysis of estrogen and progesterone receptors in fresh frozen breast tissue
• They are compatible with both Toluidine blue and Haematoxylin and Eosin rapid frozen section stains

Use:

SUPERFROST PLUS Gold Slides are inherently tissue binding, so there is no need to add sprays or adhesives to the slide.

1. Allow the SUPERFROST PLUS Gold Slides to contact the frozen section directly in the cryostat using your standard technique.
2. Place the slide horizontally on a table top (tissue side up) at room temperature for 20-30 seconds (for fresh tissue) or for 1 minute (for fixed tissue). This allows time for the revolutionary surface binding chemistry in the SUPERFROST PLUS Gold Slides to unite with the tissue section. This short time at room temperature does not cause frozen section artifact because the slides hold both nuclear and cytoplasmic tissue components, which are firmly immobilised in their place during the bonding process. The longer you allow for bonding time, the more frozen tissue will adhere to the slide.
3. Postfix the SUPERFROST PLUS Gold Slide in acetone or in an alcohol-based fixative. Postfixing in formalin is NOT recommended.
4. Stain as per your standard protocol.
5. Store the SUPERFROST PLUS Gold Slide at room temperature.
6. Your specially designed SUPERFROST PLUS Gold Slide box can be used as a storage box when empty.