{"title":"Aurion - Silver Enhancement Reagents","description":null,"products":[{"product_id":"aurion-enhancement-conditioning-solution","title":"Aurion enhancement conditioning solution","description":"\u003cp\u003eAURION Enhancement Conditioning Solcution – ECS – is a concentrated buffer solution, improving immuno gold silver staining results. Aurion ECS is supplied as a 10x concentrate and is compatible with all the Aurion reagents.\u003c\/p\u003e\u003cp\u003eDetailed information is provided in the package insert. See also Aurion Newsletter 5 as well as the info on double labelling on this website.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eConcentrated solution\u003c\/li\u003e\n\u003cli\u003eSupports ultrastructural preservation of delicate specimens\u003c\/li\u003e\n\u003cli\u003eSupports optimum silver enhancement results in single labelling by avoiding ion concentration shocks and dilution affects\u003c\/li\u003e\n\u003cli\u003eFacilitates repeated solution changes in double labelling using silver enhancement\u003c\/li\u003e\n\u003cli\u003eStraightforward method\u003c\/li\u003e\n\u003cli\u003eEconomical use\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eUsed as a 10x dilution Aurion ECS serves as a washing medium after immuno incubation and before silver enhancement (immuno procedure – wash – silver enhancement).\u003c\/p\u003e\u003cp\u003eIn addition to this application in singe labelling it is required in double labelling experiments as a washing medium between the first silver enhancement step and the second immuno incubation sequence.\u003cbr\u003e(immuno incubation1 – wash – silver enhancement1 – wash – immuno incubation 2 – wash – silver enhancement2).\u003c\/p\u003e\u003cp\u003eAurion ECS has a guaranteed shelf life of 18 months from the date of quality control analysis. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStore at 4-8°C. Do not freeze.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25568.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"100ml","offer_id":40973125386417,"sku":"EMS25830","price":203.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/EMS25830-ECS.png?v=1770942923"},{"product_id":"aurion-silver-enhancement-reagents-electron-microscopy","title":"Aurion silver enhancement reagents, electron microscopy","description":"\u003cp\u003eAURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications. The enhancement mixture has a pH of 8.1-8.2.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHigh efficiency silver enhancement\u003c\/li\u003e\n\u003cli\u003eHomogeneous particles\u003c\/li\u003e\n\u003cli\u003eFor electron microscopy\u003c\/li\u003e\n\u003cli\u003eLight insensitive\u003c\/li\u003e\n\u003cli\u003eLow viscosity solutions\u003c\/li\u003e\n\u003cli\u003eVirtually no auto nucleation\u003c\/li\u003e\n\u003cli\u003eDropping bottles, easy preparation\u003c\/li\u003e\n\u003cli\u003eMix-and-use\u003c\/li\u003e\n\u003cli\u003eEconomical use, no left-overs\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAURION R-Gent SE-EM has been tested intensively for the enhancement of AURION Ultra Small Immunogold reagents.\u003c\/p\u003e\u003cp\u003eAURION R-Gent SE-EM is available in a kit containing the following:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e30ml or 90ml of ready-to-use ENHANCER\u003c\/li\u003e\n\u003cli\u003e3ml of concentrated INITIATOR\u003c\/li\u003e\n\u003cli\u003e30ml of ACTIVATOR\u003c\/li\u003e\n\u003cli\u003e3ml empty dropping bottle, labeled “DEVELOPER”\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThe INITIATOR is a concentrated solution which must be diluted and activated before use, using the ACTIVATOR. This resulting mixture is the DEVELOPER. The now ready-to-use DEVELOPER has a shelf life of one month. It is suggested to prepare fresh DEVELOPER at least every month.\u003cbr\u003eThe undiluted solutions have a shelf life of 10 months when stored at 4°C. INITIATOR can be stored at -20°C for prolonged shelf life.\u003c\/p\u003e\u003cp\u003eFor enhancement 1-2 drops of DEVELOPER are mixed with 20 drops of ENHANCER. The typical enhancement time is between 20 and 40 minutes. Specimens may be contrasted according to standard procedures.\u003c\/p\u003e\u003cp\u003eThe kit with 30 ml of ENHANCER accomodates up to 1000 EM grid specimens. The kit with 90 ml of ENHANCER is suitable for pre-embedding immunogold labeling; the number of specimens it accomodates depends on the volume of reagent used per specimen.\u003c\/p\u003e\u003cp\u003eFor each lot, the specific enhancement activity and level of autonucleation are monitored by spectrophotometric techniques.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThe AURION R-Gent SE-EM components are stored at 4°C and allowed to reach room temperature before use. SE-EM INITIATOR may be stored at -20°C for prolonged shelf life\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25282.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"Electron microscopy \/ 30ml","offer_id":46687639109809,"sku":"JA500-033","price":547.0,"currency_code":"AUD","in_stock":true},{"title":"Electron microscopy \/ 90ml","offer_id":46687639142577,"sku":"EMS25521-90","price":906.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/ASEK-EM.jpg?v=1772587396"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/collections\/AurionLogo_080c5ff3-cef1-4e68-8325-6e185834e567.png?v=1786074826","url":"https:\/\/ems.proscitech.com.au\/collections\/aurion-silver-enhancement-reagents.oembed","provider":"EMS Australia","version":"1.0","type":"link"}