{"title":"Aurion - Gold Nanoparticles","description":null,"products":[{"product_id":"col-aurion-total-protein-stain","title":"COL-Aurion colloidal gold-based protein stain","description":"\u003cp\u003eCol-Aurion is a colloidal gold particle based total protein stain, developed for the sensitive staining of electrophoretically separated protein bands on nitrocellulose or PVDF blotting membranes. Colloidal gold particles accumulate at the site of the protein bands on the membrane, generating an intense dark red staining pattern. The total protein stain assists in assessing immunoblotting results and to evaluate the effectivity of the blotting procedure. \u003c\/p\u003e\u003cp\u003eThe negative surface charge of colloidal gold particles is responsible for their high affinity for positively charged macromolecules. This characteristic was the basis for the development of a total protein stain based on colloidal gold. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDescription:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCol-Aurion is a total protein stain consisting of a solution of coated gold particles with an average particle diameter of 15nm. The staining principle is based on the electrostatic binding of negatively charged gold particles to proteins with a positive charge present at low pH. A unique feature of Col-Aurion is the use of BSA-c to shield off the surface of the gold particles. Destabilisation of gold particles that might occur as a result of interaction with detached protein is thus prevented. The strong negative charge of BSA-c gives an additional increase in sensitivity of the stain. Col-Aurion total protein stain has a pH of 3.2 and is ready to use. For removal of surplus of weakly bound protein from the membrane, a 10 ml vial of Tween-20 is included.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCol-Aurion has a guaranteed shelf life of 18 months from the date of quality control analysis. \u003c\/p\u003e\u003cp\u003eStore at 4-8°C. Do not freeze.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25282.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"500ml","offer_id":40973123584177,"sku":"EMS25536","price":440.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/EMS25536-CPS_22b0626f-8b63-4830-b28b-a09b4583c785.jpg?v=1770942915"},{"product_id":"nanoparticles-gold-carboxyl-functionalized","title":"Aurion gold nanoparticles, carboxyl functionalised","description":"\u003cp\u003eAurion gold nanoparticles, carboxyl functionalised, are gold nanoparticles with polyethylene glycol-carboxyl molecules attached to their surface.\u003c\/p\u003e\u003cp\u003eAurion Gold Nanoparticles (Carboxyl Functionalised) are polyethylene glycol-carboxyl stabilised gold nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm. They are prepared using in-house developed production protocols giving the product a narrow size distribution, long term stability and optimal conjugation properties. Biomolecules having primary amines available can be covalently conjugated using EDC\/sulfo-NHS chemistry.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eCarboxyl (COOH) functionalised\u003c\/li\u003e\n\u003cli\u003eParticle sizes 6, 10, 15 and 25nm\u003c\/li\u003e\n\u003cli\u003eBind to primary amino amines in target molecule\u003c\/li\u003e\n\u003cli\u003eConjugation using EDC\/sulfo-NHS chemistry\u003c\/li\u003e\n\u003cli\u003eRecommended for conjugation of small ligands\u003c\/li\u003e\n\u003cli\u003eDetailed conjugation protocol\u003c\/li\u003e\n\u003cli\u003eReady and easy to use, long term stability\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eBiomolecules that are too small to be conjugated to Gold Nanoparticles via adsorption can be covalently linked to Carboxyl-functionalised Gold Nanoparticles. Primary amines are present in e.g. the N-terminal side of peptides and in the side group of the amino acid lysine. The conjugation relies on well known and proven EDC\/sulfo-NHS chemistry. EDC (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) is a water soluble carbodiimide which transforms the carboxyl groups on the gold to an active ester in the presence of sulfo-NHS (N-Hydroxy-sulfosucciimide, sodium salt). These sulfo-NHS esters are relatively stable in acidic environment and couple rapidly to the amine(s) in the target molecules. Aurion Gold Nanoparticles (Carboxyl Functionalised) are prepared according to a unique protocol, warranting narrow size distribution, long term stability and optimal conjugation properties. They are available in size ranges 6, 10, 15 and 25nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25nm particle size sols is less than 12%, whereas the 10 and 15nm size sols show less then 10% variation. Actual lot specifications (size, variation and expiry date) are indicated on the accompanying package insert. Aurion Gold Nanoparticles (Carboxyl Functionalised) are supplied in 10mM MES buffer, pH 5.0. Package size: 4x5 ml of high quality Carboxyl-functionalised Gold Nanoparticles at an OD520nm = 1.0.\u003c\/p\u003e\u003cp\u003eAurion Gold Nanoparticles (Carboxyl Functionalised) have a guaranteed shelf life of 12 months from the date of quality control analysis. \u003c\/p\u003e\u003cp\u003eThese are ordered fresh.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"6nm \/ 4 x 5ml","offer_id":40975701573809,"sku":"EMS25600","price":522.0,"currency_code":"AUD","in_stock":true},{"title":"10nm \/ 4 x 5ml","offer_id":40975701606577,"sku":"EMS25601","price":522.0,"currency_code":"AUD","in_stock":true},{"title":"15nm \/ 4 x 5ml","offer_id":40975701639345,"sku":"EMS25602","price":522.0,"currency_code":"AUD","in_stock":true},{"title":"25nm \/ 4 x 5ml","offer_id":40975701672113,"sku":"EMS25603","price":522.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/EMS25600-GS.png?v=1758757808"},{"product_id":"aurion-gold-nanoparticles-gold-sols","title":"Aurion gold nanoparticles, gold sols","description":"\u003cp\u003eAurion ST-US is based on Ultra Small atomic gold clusters (number of atoms\/cluster \u0026lt;30) having an average diameter of approximately 0.8nm. The Ultra Small particles have been stabilised with Bovine Serum Albumin fragments which may be covalently bound to macromolecules by e.g., glutaraldehyde cross-linking.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eBased on ultra small atomic gold clusters\u003c\/li\u003e\n\u003cli\u003eAverage diameter 0.8nm\u003c\/li\u003e\n\u003cli\u003eBovine Serum Albumin stabilised\u003c\/li\u003e\n\u003cli\u003eFor covalent conjugation using glutaraldehyde\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25260.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"6nm \/ 100ml","offer_id":46689957052593,"sku":"JA406-011","price":545.0,"currency_code":"AUD","in_stock":true},{"title":"10nm \/ 100ml","offer_id":46689957085361,"sku":"JA410-011","price":545.0,"currency_code":"AUD","in_stock":true},{"title":"15nm \/ 100ml","offer_id":46689957118129,"sku":"JA415-011","price":545.0,"currency_code":"AUD","in_stock":true},{"title":"25nm \/ 100ml","offer_id":46689957150897,"sku":"JA425-011","price":545.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/JA400.png?v=1772662849"},{"product_id":"aurion-ultra-small-gold-nanoparticles-gold-sols","title":"Aurion ultra small gold nanoparticles, gold sols","description":"\u003cp\u003eAurion ST-US is based on Ultra Small atomic gold clusters (number of atoms\/cluster \u0026lt;30) having an average diameter of approximately 0.8nm. The Ultra Small particles have been stabilised with Bovine Serum Albumin fragments which may be covalently bound to macromolecules by e.g., glutaraldehyde cross-linking.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eBased on ultra small atomic gold clusters\u003c\/li\u003e\n\u003cli\u003eAverage diameter 0.8nm\u003c\/li\u003e\n\u003cli\u003eBovine Serum Albumin stabilised\u003c\/li\u003e\n\u003cli\u003eFor covalent conjugation using glutaraldehyde\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25260.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"UltraSmall \/ 20ml","offer_id":46689957183665,"sku":"JA400-011","price":595.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/files\/JA400_4da8f16f-1483-4aaf-a8dc-d2a118290d4c.png?v=1772662854"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2035\/7759\/collections\/AurionLogo_6df36c66-33c8-4b79-92c2-5305b10ae190.png?v=1786074722","url":"https:\/\/ems.proscitech.com.au\/collections\/aurion-gold-nanoparticles.oembed","provider":"EMS Australia","version":"1.0","type":"link"}